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Tris is a very important biological buffer, commonly used as a combo with hydroxymethyl aminomethane, keeping pH at 7.0-9.1 stably. It is sensitive to temperature and concentration. The pH value of Tris based aqueous solution is about 10.5. Hydrochloric... moreTris is a very important biological buffer, commonly used as a combo with hydroxymethyl aminomethane, keeping pH at 7.0-9.1 stably. It is sensitive to temperature and concentration. The pH value of Tris based aqueous solution is about 10.5. Hydrochloric acid is usually added to adjust the pH value to the desired value. EDTA is usually added to Tris hydrochloric acid buffer to make "TE buffer".
#20 #mm #tris #buffer
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Good's buffer has the following characteristics: 1. High water solubility; 2. Low cell membrane permeability; 3. stable acid-base dissociation constant; 4. Low metal chelating capacity; 5. High chemical stability; 6. Low absorption spectra in ultraviolet... moreGood's buffer has the following characteristics: 1. High water solubility; 2. Low cell membrane permeability; 3. stable acid-base dissociation constant; 4. Low metal chelating capacity; 5. High chemical stability; 6. Low absorption spectra in ultraviolet and visible region;7. Low salt effect; 8. Easy to make high purity salt. They are essential raw materials for in vitro diagnostic reagents in the process of production.
#tris #hydroxymethyl #aminomethane
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